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CAS No:   22150-76-1



6-Biopterin  (L-erythro-Biopterin)


Synonyms: L-Biopterin, 2-Amino-4-Hydroxy-6-(1,2-Dihydroxypropyl) Pteridine


Molecular Formula:C9H11N5O3


CAS Number:22150-76-1


Formula Weight:237.2


Description:White or light yellow powder


Biochemical functions:Biopterin has two isomers (D- and L-biopterin). We sell only L-Biopterin. The hydrogenated forms of biopterin i.e. dihydrobiopterin and tetrahydrobiopterin are involved in many important biochemical functions in the human body, among which are the hydroxylation of tyrosine, phenylalanine and tryptophan.


Solubility:Bioptein is very slightly soluble in water. The solubility of biopterin is about 0.01 g per 100 g of water (220C). Concentrated solutions of biopterin may be prepared using 0.05M NaOH. Ultrasonication may be used to improve dissolution.


Analytical methods:

        HPLC conditions:  Column: Waters Spherisorb S5 ODS1

                          Eluant: 10mM Na2HPO4 PH 6-methanol, (19:1)

                          Flow rate: 1ml/min

                          Wavelength: 254nm

                          Solution: 2mg/ml 0.05 M NaOH

        TLC conditions:    Stationary phase: cellulose

                          Eluant: water


Specifications: Purity: HPLC   >98.0%

                   TLC    one blue fluorescent spot at 366nm


Stability:Bioptein is light sensitive. Biopterin contains half a mole of water. It can be stored for several years, dry, in tightly closed bottle coloured , at –200C or colder.


Storage: Keep the powder dry in tightly closed bottle coloured , at –200C. Biopterin can be transported without the use of dry ice. In tightly closed bottle, wrapped in aluminium foil, it is stable at ambient temperature for several months.


Uses:Biopterin is an important  standard for analytical work involving tetrahydrobiopterin and dihydrobiopterin. It is not suitable for the addition to nutritional supplements .


Safety information: Bioptein is known to be safe and there are no special precautions required in handling this product.


Remarks: The information in this publication is based on our current knowledge and experience. It does not relieve users or processors from carrying out their own precautions and tests.

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